ABSTRACT:

The global economic importance of poultry for the production of meat, eggs, and related by-products, either through commercial poultry farms or by small-family owned producers, is undeniably crucial. Mycoplasma gallisepticum (MG) is the causative agent of highly transmissible and persistent Chronic Respiratory Disease (CRD). The clinical and economic burden of CRD causes substantial losses to the poultry industry and is particularly devastating to smaller producers. Imported vaccines are costly, difficult to obtain, and less effective due to frequently emerging mutant strains. The present study aimed to synthesize an oil-based inactivated vaccine using local isolates of Mycoplasma gallisepticum (MG) as a strategic control measure. This vaccine has the potential to serve as an effective alternative to currently imported vaccines. MG strains were isolated from infected broilers and obtained from poultry farms located in the Tret, Tarli, and Sehala areas of Islamabad. Effective Inactivation Dose (EID 70) of the cultures was calculated. The antigen was inactivated by 0.1% formalin and then washed in sterile PBS. The vaccine was prepared in montanide oil in 40:60 ratios. A commercial ELISA kit detected an antibody titer. Three vaccines, A, B, and C, were prepared from three different isolates. Two weeks old broilers were divided into 3 groups, each containing 10 birds and vaccinated. Unvaccinated birds were maintained as negative and positive control groups. Post-inoculation antibody titer after 15 and 30 days was 4 and 7, respectively. Birds were challenged with the EID 70 dose of respective antigens and an antigen mixture. The vaccines prepared from local isolates were found to be effective against CRD. The current study proves the effectiveness of locally produced vaccines against CRD; however, a larger scale is required in this respect.0

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