Plant bioactivities encompass the range of biological effects due to compounds derived from plants or plant-based products on living organisms. These bioactivities are typically due to the bioactive compounds of plants, such as alkaloids, flavonoids, terpenoids, polyphenols, and other secondary metabolites. These compounds can influence various physiological processes and exhibit medicinal, nutritional, and therapeutic properties. Typha elephantina is a medicinally potent plant. The study aimed to qualify the bioactive compounds from the methanolic extract of Typha elephantina through the advanced Tandem mass spectrometry technique. After characterization, the data were interpreted based on precursor and product ions from MS, MS₂ and MS₃ Analysis. A total of ten compounds were identified: Isomagnolol, (8E,10E,12E)-octadic-8,10,12-trienoic acid, Linoleic acid, Wogonin, 9E,11E-oxooctadeca-9,11-dienoic acid, 9-hydroxy-10,12-octadecadienoic acid, 18 hydroxy oleic acid, Robustaflavone, 3′, 3”’-Binaringenin and 7-O-[6”’-O-propenyl]-Allosyl(1-2) Glucoside. These bioactive compounds play essential roles in plant defense, ecological interactions, and stress responses in pharmaceutical, agricultural, and food industries, focusing on their antioxidant, antimicrobial, anti-inflammatory, and anticancer properties. Exploring plant secondary metabolites remains a promising avenue for developing novel therapeutic agents and enhancing ecological sustainability.

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