Research Article | Open Access Volume 2 | Issue 3 | Paper 08 | https://doi.org/10.15228/2012.v02.i03.p08 |
Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography
A.J. Abdulghani
Department of chemistry, College of Sciences, University of Baghdad, Jaderiya, Iraq
H. H. Jasim
Department of chemistry, College of Sciences, Al-Mustansiria University, Baghdad, Iraq
A.S. Hassan
Department of chemistry, College of Sciences, Al-Mustansiria University, Baghdad, Iraq
| Received 23 Mar, 2012 | Accepted 23 Jun, 2012 | Published 23 Dec, 2012 |
ABSTRACT:
The determination amoxicillin, ampicillin and cephalexin was studied by complexation of the antibiotics with Au(III) and Hg(II) ions in bulk and pharmaceutical preparations using uv-visible spectrophotometry, atomic absorption, and HPLC techniques. Optimum conditions for complex formation were fixed at pH 4 and (2-4) for Au(III) and Hg(II) complexes respectively, heating temperature at (60°C) and heating time for (10 minute). All complexes were extracted from aqueous solution with benzyl alcohol prior to measurements except in the case of HPLC. The L:M ratios for all complexes were determined and stability constants were calculated using mole ratio method. The Beer’s law was obeyed over the concentration range (5-60) and 5-50 µg/ml of antibiotics for Au(III) and Hg(II) complexes using colorimetric method and (1-25 µg/ml) of Au(III) for FAAS. The linearity for HPLC method was (10-110 and 10-120 µg/ml) respectively. The correlation coefficients (r) were (0.9981-0.9997). Generally, the highest sensitivity was recorded by FAAS.
How to Cite this paper?
APA-7 Style
Abdulghani, A.J., Jasim, H.H., Hassan, A.S. (2012). Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pakistan Journal of Chemistry, 2(3), 150-160. https://doi.org/10.15228/2012.v02.i03.p08
ACS Style
Abdulghani, A.J.; Jasim, H.H.; Hassan, A.S. Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pak. J. Chem. 2012, 2, 150-160. https://doi.org/10.15228/2012.v02.i03.p08
AMA Style
Abdulghani AJ, Jasim HH, Hassan AS. Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pakistan Journal of Chemistry. 2012; 2(3): 150-160. https://doi.org/10.15228/2012.v02.i03.p08
Chicago/Turabian Style
Abdulghani, A., J., H. H. Jasim, and A. S. Hassan. 2012. “Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography” Pakistan Journal of Chemistry 2, no. 3: 150-160. https://doi.org/10.15228/2012.v02.i03.p08
This work is licensed under a Creative Commons Attribution 4.0 International License.