Contamination of drinking water especially with heavy metals is now a major issue from both the public health and the environmental health perspectives. In present work we are reporting a multivariate study for the concentrations of Arsenic and Iron in groundwater n=334 collected from Gambat, Khairpur, Sindh, Pakistan during year 2008. The analysis was performed using Hydride Generator Atomic Absorption Spectrometry (HG-ASS) Perkin Elmer A-100 coupled with MHS-15. Arsenic and Iron were evaluated in hand pump and tube well water sample with detection limit 0.02µgL^-1 and 01µgL^-1 respectively. The level of arsenic was found in hand pump and tube well water ranged from <0.01 to 126µgL^-1 and <0.01-38 µgl^-1 respectively. While level of Iron was found in the rage of <0.004^-1.6mgL^-1 and <0.004^-1.5mgL^-1 in hand pump and tube well groundwater respectively. It has observed that in most of the samples level of these both elements were above than the maximum permissible level of World Health Organization.