The Determination of Cetirizine in The Presence of Cephalosporins Through Novel Rp-Hplc Method and Application in Human Serum

ABSTRACT:

The current article described a completely validated RP-HPLC method that described simultaneous quantification of cetirizine in the presence of any of the three cephalosporins, i.e., ceftriaxone or cephalexin or cefotaxime while amlodipine (calcium channel blocker) used as an internal standard. Cetirizine, a widely prescribed drug as allergy medication. It is also co-administered with cephalosporins. The methanol: acetonitrile: water in a ratio of 65:5:30 with a pH to 3.2 with phosphoric acid mobile phase was selected to keep the method least expensive. All the validation parameters such as accuracy, precision, repeatability, suitability were checked and were found to be friendly according to ICH guidelines. The LOD and LOQ in each case were evaluated and for cetirizine were found to be 0.001 and 0.003 µgmL^-1 , ceftriaxone 0.06 and 0.211 µgmL^-1 , cephalexin 0.016 and 0.054 µgmL^-1 , cefotaxime 0.012 and 0.04 µgmL^-1 , respectively. The method was found to possess applications for clinical purposes and pharmaceutical labs.