Research Article | Open Access Volume 3 | Issue 2 | Paper 02 | doi.org/10.15228/2013.v03.i02.p02 |
Y. Sunandamma
Department of Chemistry, Acharya Nagarjuna University, Guntur, Andra Pradesh, India
V. Phani Kumar
Department of Chemistry, Acharya Nagarjuna University, Guntur, Andra Pradesh, India
| Received 27 Aug, 2012 | Accepted 14 Feb, 2013 | Published 10 Apr, 2013 |
ABSTRACT:
A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Fulvestrant in tablet dosage form. Isocratic elution at a flow rate of 1 ml/min was employed on a symmetry Chromosil C18 (250×4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of Methanol: 1% OPA: 85:15 % (V/V). The UV detection wavelength was 243 nm and 20µl sample was injected. The retention time for Fulvestrant was 5.3 min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Fulvestrant in tablet dosage form and bulk drug.
How to Cite this paper?
APA-7 Style
Sunandamma, Y., Kumar, V.P. (2013). A Novel RP-HPLC Method for the Quantification of Fulvestrant in Formulations
. Pakistan Journal of Chemistry, 3(2), 51-55. https://doi.org/10.15228/2013.v03.i02.p02
ACS Style
Sunandamma, Y.; Kumar, V.P. A Novel RP-HPLC Method for the Quantification of Fulvestrant in Formulations
. Pak. J. Chem. 2013, 3, 51-55. https://doi.org/10.15228/2013.v03.i02.p02
AMA Style
Sunandamma Y, Kumar VP. A Novel RP-HPLC Method for the Quantification of Fulvestrant in Formulations
. Pakistan Journal of Chemistry. 2013; 3(2): 51-55. https://doi.org/10.15228/2013.v03.i02.p02
Chicago/Turabian Style
Sunandamma, Y., and V. Phani Kumar. 2013. “A Novel RP-HPLC Method for the Quantification of Fulvestrant in Formulations
” Pakistan Journal of Chemistry 3, no. 2: 51-55. https://doi.org/10.15228/2013.v03.i02.p02
This work is licensed under a Creative Commons Attribution 4.0 International License.