Research Article | Open Access Volume 2 | Issue 4 | Paper 04 | doi.org/10.15228/2012.v02.i04.p04 |
H. Bokhari
Department of Chemistry Post Graduate Islamia College Cooper road Lahore
M.R. Khan
Department of Chemistry Government College University Lahore
| Received 29 Aug, 2012 | Accepted 27 Sep, 2012 | Published 05 Nov, 2012 |
ABSTRACT:
Proteolytic enzymes can immobilize on different materials to constitute systems for continuous hydrolysis of protein substrates. They are mostly immobilized by binding some matrices filled in chromatographic columns and continuous proteolysis is accomplished by subsequent passing of the buffered substrates through the column bed from top to bottom and collecting of hydrolyzed products eluting out of the column from the bottom. Immobilization of the protease of Carica Papaya was carried out on DEAE-cellulose. The percentage of immobilization was found to be 67%.The life span of the enzyme immobilized on DEAE-cellulose was 30 days.
How to Cite this paper?
APA-7 Style
Bokhari, H., Khan, M.R. (2012). Immobilization of the Protease of Carica papaya on DEAE-cellulose. Pakistan Journal of Chemistry, 2(4), 178-182. https://doi.org/10.15228/2012.v02.i04.p04
ACS Style
Bokhari, H.; Khan, M.R. Immobilization of the Protease of Carica papaya on DEAE-cellulose. Pak. J. Chem. 2012, 2, 178-182. https://doi.org/10.15228/2012.v02.i04.p04
AMA Style
Bokhari H, Khan MR. Immobilization of the Protease of Carica papaya on DEAE-cellulose. Pakistan Journal of Chemistry. 2012; 2(4): 178-182. https://doi.org/10.15228/2012.v02.i04.p04
Chicago/Turabian Style
Bokhari, H., and M. R. Khan. 2012. “Immobilization of the Protease of Carica papaya on DEAE-cellulose” Pakistan Journal of Chemistry 2, no. 4: 178-182. https://doi.org/10.15228/2012.v02.i04.p04
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