Research Article | Open Access Volume 2 | Issue 3 | Paper 08 doi.org/10.15228/2012.v02.i03.p08 |
A.J. Abdulghani
Department of chemistry, College of Sciences, University of Baghdad, Jaderiya, Iraq
H. H. Jasim
Department of chemistry, College of Sciences, Al-Mustansiria University, Baghdad, Iraq
A.S. Hassan
Department of chemistry, College of Sciences, Al-Mustansiria University, Baghdad, Iraq
| Received 02 Aug, 2012 | Accepted 15 Sep, 2012 | Published 30 Sep, 2012 |
ABSTRACT:
The determination amoxicillin, ampicillin and cephalexin was studied by complexation of the antibiotics with Au(III) and Hg(II) ions in bulk and pharmaceutical preparations using uv-visible spectrophotometry, atomic absorption, and HPLC techniques. Optimum conditions for complex formation were fixed at pH 4 and (2-4) for Au(III) and Hg(II) complexes respectively, heating temperature at (60°C) and heating time for (10 minute). All complexes were extracted from aqueous solution with benzyl alcohol prior to measurements except in the case of HPLC. The L:M ratios for all complexes were determined and stability constants were calculated using mole ratio method. The Beer’s law was obeyed over the concentration range (5-60) and 5-50 µg/ml of antibiotics for Au(III) and Hg(II) complexes using colorimetric method and (1-25 µg/ml) of Au(III) for FAAS. The linearity for HPLC method was (10-110 and 10-120 µg/ml) respectively. The correlation coefficients (r) were (0.9981-0.9997). Generally, the highest sensitivity was recorded by FAAS.
How to Cite this paper?
APA-7 Style
Abdulghani, A.J., Jasim, H.H., Hassan, A.S. (2012). Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pakistan Journal of Chemistry, 2(3), 150-160. https://doi.org/10.15228/2012.v02.i03.p08
ACS Style
Abdulghani, A.J.; Jasim, H.H.; Hassan, A.S. Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pak. J. Chem. 2012, 2, 150-160. https://doi.org/10.15228/2012.v02.i03.p08
AMA Style
Abdulghani AJ, Jasim HH, Hassan AS. Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography. Pakistan Journal of Chemistry. 2012; 2(3): 150-160. https://doi.org/10.15228/2012.v02.i03.p08
Chicago/Turabian Style
Abdulghani, A., J., H. H. Jasim, and A. S. Hassan. 2012. “Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid
Chromatography” Pakistan Journal of Chemistry 2, no. 3: 150-160. https://doi.org/10.15228/2012.v02.i03.p08
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