{"id":4156,"date":"2026-06-25T14:17:34","date_gmt":"2026-06-25T14:17:34","guid":{"rendered":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/?p=4156"},"modified":"2026-06-30T11:46:00","modified_gmt":"2026-06-30T11:46:00","slug":"anst-0826","status":"publish","type":"post","link":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/2026\/06\/25\/anst-0826\/","title":{"rendered":"Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica\u00a0Leaf Extracts Obtained Using Different Solvents"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"4156\" class=\"elementor elementor-4156\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-09498ad e-flex e-con-boxed e-con e-parent\" data-id=\"09498ad\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-f76763f e-con-full e-flex e-con e-child\" data-id=\"f76763f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-db9a352 elementor-widget elementor-widget-text-editor\" data-id=\"db9a352\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td><div><strong>Research Article | Open Access<\/strong><\/div><div class=\"item doi\"><span class=\"value\">Volume 6 | Issue 03-4\u00a0 | Paper 08 |\u00a0 <a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2026\/06\/ANST.v06.i3-4.p08.pdf\">https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08<\/a><\/span><\/div><\/td><\/tr><\/tbody><\/table><h4><strong><b>Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of <\/b><\/strong><strong><em><b><i>Aerva persica<\/i><\/b><\/em><\/strong><strong><b>\u00a0Leaf Extracts Obtained Using Different Solvents<\/b><\/strong><\/h4><div class=\"row\"><div class=\"main_entry\"><p><strong>Zainab Bibi<\/strong><br \/>Department of Zoology, Wildlife &amp; Fisheries, University of Agriculture, Faisalabad, Pakistan<\/p><p><strong>Saima Naz<\/strong><br \/>Department of Chemistry, University of Education, Faisalabad Campus, Pakistan<\/p><p><strong>Kanz ul Eman<\/strong><br \/>Institute of Biochemistry, Biotechnology and Bioinformatics, the Islamia University of Bahawalpur, Bahawalpur-63100,\u00a0Pakistan<\/p><p><a href=\"https:\/\/orcid.org\/0009-0001-0204-5451\" target=\"_blank\" rel=\"noopener\"><strong>Fania Rashid<\/strong><\/a><br \/>Department of Chemistry, Government College University Lahore, Pakistan<\/p><p><strong>Ayeza Sana<\/strong><br \/>Department of Pharmacy, The Islamia University of Bahawalpur, Pakistan<\/p><p><strong>Areeba Samin<\/strong><br \/>Institute of Microbiology and Molecular Genetics, University of Punjab, Lahore, Pakistan<\/p><p><strong>Umme Rubab<\/strong><br \/>Department of Chemistry, University of Education, Faisalabad Campus, Pakistan<\/p><p><strong>Kiran Qamar<\/strong><br \/>Department of Chemistry, University of Education, Faisalabad Campus, Pakistan<\/p><p><strong>Shahzaib Ishfaq<\/strong><\/p><\/div><p>Department of Chemistry, University of Education, Faisalabad Campus, Pakistan<\/p><div class=\"main_entry\"><table border=\"0\" width=\"93%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td align=\"center\" valign=\"top\"><strong>Received<\/strong><br \/>04 May, 2026<\/td><td align=\"center\" valign=\"top\"><strong>Accepted<\/strong><br \/>18 Jun, 2026<\/td><td align=\"center\" valign=\"top\"><strong>Published<\/strong><br \/>25 Jun, 2026<\/td><\/tr><\/tbody><\/table><div class=\"item abstract\"><p align=\"justify\"><strong>ABSTRACT:<\/strong><\/p><p>This study investigates the phytochemical profile and in vitro biological activities of <em><i>Aerva persica<\/i><\/em>\u00a0leaf extracts obtained using solvents of varying polarity, including <em><i>n<\/i><\/em>-hexane, chloroform, 80% ethanol, <em><i>n<\/i><\/em>-butanol, and 80% methanol. Quantitative analysis revealed that the 80% ethanol extract contained the highest total phenolic and flavonoid contents, indicating superior extraction efficiency for antioxidant compounds. Among all tested samples, the 80% ethanol extract exhibited the strongest antioxidant potential, with the highest DPPH radical-scavenging activity (82.77%), notable reducing power, and inhibition of lipid peroxidation. Antimicrobial screening demonstrated significant antibacterial and antifungal effects, particularly in 80% ethanol extracts, with measurable zones of inhibition against both Gram-positive and Gram-negative bacteria and fungal strains. The 80% ethanolic extract also showed the highest thrombolytic activity (45.72%), suggesting moderate clot-dissolving potential. Hemolytic assays indicated generally low cytotoxicity across extracts, with chloroform showing comparatively higher hemolysis. Overall, <em><i>A. persica<\/i><\/em>\u00a0leaves represent a promising source of bioactive phytochemicals with antioxidant, antimicrobial, and thrombolytic potential for further pharmacological exploration.<\/p><p><strong><b>Keywords:<\/b><\/strong>\u00a0Biological activity, Thrombolytic activity, Hemolytic activity, Phenolics, Flavonoids, Antioxidant<\/p><p><strong>How to Cite this paper?<\/strong><\/p><\/div><div class=\"item abstract\"><div class=\"csl-entry\"><p><strong>APA-7 Style<\/strong><br \/>Bibi Z., Naz S., Eman K., Rashid F., Sana A., Samin A., Rubab U., Qamar K., Ishfaq S., (2026) Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of <em><i>Aerva persica<\/i><\/em> Leaf Extracts Obtained Using Different Solvents\u00a0<a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2026\/06\/ANST.v06.i3-4.p08.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 6(3-4), 55-63<\/a>. https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08<\/p><p><strong>ACS Style<\/strong><br \/>Z. Bibi, S. Naz, K. Eman, F. Rashid, A. Sana, A. Samin, U. Rubab, K. Qamar, S. Ishfaq, <em>Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica Leaf Extracts Obtained Using Different Solvents\u00a0<\/em><a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2026\/06\/ANST.v06.i3-4.p08.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 6(3-4), 55-63<\/a>. https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08<\/p><p><strong>AMA Style<\/strong><br \/>Z. Bibi; S. Naz; K. Eman; F. Rashid; A. Sana; A. Samin; U. Rubab; K. Qamar; S. Ishfaq; <em>Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica Leaf Extracts Obtained Using Different Solvents\u00a0<\/em><a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2026\/06\/ANST.v06.i3-4.p08.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 6(3-4), 55-63<\/a>. https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08<\/p><p><strong>Chicago\/Turabian Style<\/strong><br \/>Z Bibi, S Naz, K Eman, F Rashid, A Sana, A Samin, U Rubab, K Qamar, S Ishfaq, <em>Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica Leaf Extracts Obtained Using Different Solvents (2026) <\/em><a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2026\/06\/ANST.v06.i3-4.p08.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 6(3-4), 55-63<\/a>. https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08<\/p><\/div><\/div><div class=\"item copyright\"><a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\"><img decoding=\"async\" src=\"https:\/\/i.creativecommons.org\/l\/by\/4.0\/88x31.png\" alt=\"Creative Commons License\" \/><\/a><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-db7da9d e-con-full e-flex e-con e-child\" data-id=\"db7da9d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Research Article | Open AccessVolume 6 | Issue 03-4\u00a0 | Paper 08 |\u00a0 https:\/\/doi.org\/10.15228\/ANST.2026.v06.i03-4.p08 Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica\u00a0Leaf Extracts Obtained Using Different Solvents Zainab BibiDepartment of Zoology, Wildlife &amp; Fisheries, University of Agriculture, Faisalabad, Pakistan Saima NazDepartment of Chemistry, University of Education, Faisalabad Campus, Pakistan Kanz ul [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[34,36],"tags":[],"class_list":["post-4156","post","type-post","status-publish","format-standard","hentry","category-34","category-issue-3-4-2026"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Comparative Evaluation of Antioxidant, Antimicrobial, Thrombolytic, and Hemolytic Activities of Aerva persica\u00a0Leaf Extracts Obtained Using Different Solvents - The Journal Advances of Nutrition 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