{"id":3095,"date":"2024-12-25T22:25:00","date_gmt":"2024-12-25T22:25:00","guid":{"rendered":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/?p=3095"},"modified":"2025-02-13T22:30:55","modified_gmt":"2025-02-13T22:30:55","slug":"anst-1024","status":"publish","type":"post","link":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/2024\/12\/25\/anst-1024\/","title":{"rendered":"Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of Ajuga bracteosa"},"content":{"rendered":"\t\t
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Research Article | Open Access<\/strong><\/div>
Volume 4 | Issue 3-4 | Paper 10 |\u00a0 https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10<\/a><\/span><\/div><\/td><\/tr><\/tbody><\/table>

Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Microropagation of Ajuga Bracteosa\u00a0<\/h4>

Mazhar Ali\u00a0<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Abdul Mateen Khattak<\/span><\/p>

The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan<\/p>

Naveed Ahmed<\/span><\/p>

The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan<\/p>

Kamran Rauf<\/span><\/p>

The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan<\/p>

Irfan Ullah<\/span><\/p>

The University of Agriculture Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Shahid Ali<\/span><\/p>

National Agricultural Research Centre, Islamabad-Pakistan<\/p>

Aamir Khan<\/span><\/p>

The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan<\/p>

Muhammad Abdul Rauf<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Khalil Ur Rahman<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Javed Rahman<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Wasim Bilal<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Mohammad Ilyas<\/span><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>

Farooq<\/span><\/a><\/p>

Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan<\/p>
Received<\/strong>
16 Oct, 2024<\/td>		Accepted<\/strong>
09 Nov, 2024<\/td>		Published<\/strong>
15 Nov, 2024<\/td><\/tr><\/tbody><\/table>

ABSTRACT:<\/p>

Identification and conservation of endangered plant species are essential for safeguarding the flora of the region.\u00a0Ajuga bracteosa\u00a0<\/em>(A. bracteosa<\/em>), found in Pakistan\u2019s North West, holds immense medicinal potential due to its diverse active ingredients. However, unskilled overharvesting of this plant by uprooting entire herbs has drastically reduced its propagules, worsening its endangered status. The current study was conducted to optimize phytohormone levels in Murashige and Skoog media for micropropagation of\u00a0A. bracteosa<\/em>. In vitro micropropagation is an effective conservation technique for\u00a0A. bracteosa<\/em>\u00a0due to its ability to preserve genetic diversity, enable rapid multiplication, produce disease-free plants, and facilitate long-term storage and utility in medicine. The study was divided into two experiments. In the first experiment, shoot organogenesis was studied in callus explants in the media containing 6-Benzylaminopurine (BAP) alone and combined with Auxins or Gibberellic Acid (GA3). In the second experiment, these shoots were studied for root organogenesis in the media containing auxins Indole-3-acetic acid (IAA), Indole-3- butyric acid (IBA) and Naphthaleneacetic acid (NAA) at two levels (0.5 and 1.0 mg L-1). In the first experiment, the medium containing BAP+NAA (3+1 mg L-1) induced early and 100% shoots and 13.8 shoot explants-1\u00a0with 4.1 cm length. In the second experiment, NAA (0.5 mg L-1) induced the roots early and 100% with 7.2 roots shoot-1\u00a0and 3.6 cm length. The results of the present study suggest that, for better regeneration of shoots, BAP+NAA (3+1 mg L-1) should be used in the medium, while NAA (0.5 mg L-1) is required to be added to the medium for better root regeneration.<\/p><\/div><\/div><\/div>

How to Cite this paper?<\/h3>

APA-7 Style<\/strong>
Ali, M., Khattak, A.M., Ahmed, N., Rauf, K., Ullah, I., Ali, S., Khan, A., Rauf, M.A., Ur Rahman, K., Rahman, J., Bilal, W., Ilyas, M., , F. (2024). Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of\u00a0Ajuga bracteosa<\/i>.\u00a0Journal Advances of Nutrition Science and Technology<\/em>, 4(3-4), 85-90<\/a>. https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10<\/p>

ACS Style<\/strong>
Ali, M.; Khattak, A.M.; Ahmed, N.; Rauf, K.; Ullah, I.; Ali, S.; Khan, A.; Rauf, M.A.; Ur Rahman, K.; Rahman, J.; Bilal, W.; Ilyas, M.; , F. Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of\u00a0Ajuga bracteosa<\/i>.\u00a0J. Adv. Nutri. Sci. Tech.<\/em>\u00a02024, 4, 85-90<\/a>. https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10<\/p>

AMA Style<\/strong>
Ali M, Khattak AM, Ahmed N, Rauf K, Ullah I, Ali S, Khan A, Rauf MA, Ur Rahman K, Rahman J, Bilal W, Ilyas M, F. Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of\u00a0Ajuga bracteosa<\/i>.\u00a0Journal Advances of Nutrition Science and Technology<\/em>. 2024; 4(3-4): 85-90<\/a>. https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10<\/p>

Chicago\/Turabian Style<\/strong>
Ali, Mazhar, Abdul Mateen Khattak, Naveed Ahmed, Kamran Rauf, Irfan Ullah, Shahid Ali, Aamir Khan, Muhammad Abdul Rauf, Khalil Ur Rahman, Javed Rahman, Wasim Bilal, Mohammad Ilyas, and Farooq . 2024. \u201cOptimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of\u00a0Ajuga bracteosa<\/i>\u201d\u00a0Journal Advances of Nutrition Science and Technology<\/em>\u00a04, no. 3-4: 85-90<\/a>. https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/article>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Research Article | Open AccessVolume 4 | Issue 3-4 | Paper 10 |\u00a0 https:\/\/doi.org\/10.15228\/ANST.2024.v04.i03-4.p10 Optimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Microropagation of Ajuga Bracteosa\u00a0 Mazhar Ali\u00a0 Agricultural Research Institute, Mingora, Swat, Khyber Pakhtunkhwa, Pakistan Abdul Mateen Khattak The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan Naveed Ahmed The University of Agriculture […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3095","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"yoast_head":"\nOptimizing Phytohormone Combinations in Murashige and Skoog Media for Enhanced Micropropagation of Ajuga bracteosa - The Journal Advances of Nutrition Science 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