{"id":273,"date":"2021-06-21T17:17:00","date_gmt":"2021-06-21T17:17:00","guid":{"rendered":"https:\/\/chempublishers.com\/anst\/?p=273"},"modified":"2025-06-05T16:37:00","modified_gmt":"2025-06-05T16:37:00","slug":"anst-0721","status":"publish","type":"post","link":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/2021\/06\/21\/anst-0721\/","title":{"rendered":"Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0Malva sylvestris (Pick-cheese)\u00a0leaf extracts"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"273\" class=\"elementor elementor-273\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-548bda1 e-flex e-con-boxed e-con e-parent\" data-id=\"548bda1\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-36d77f8 e-con-full e-flex e-con e-child\" data-id=\"36d77f8\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1ddbca5 elementor-widget elementor-widget-text-editor\" data-id=\"1ddbca5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td><div><strong>Research Article | Open Access<\/strong><\/div><div class=\"item doi\"><span class=\"value\">Volume 1 | Issue 2\u00a0 | Paper 02 |\u00a0 <a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2024\/12\/ANST-v1-i2-Paper-02-2.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02<\/a><\/span><\/div><\/td><\/tr><\/tbody><\/table><article class=\"obj_article_details\"><h1 class=\"page_title\">Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0<i>Malva sylvestris (Pick-cheese)<\/i>\u00a0leaf extracts<\/h1><div class=\"row\"><div class=\"main_entry\"><p><span class=\"name\">Saima Naz<\/span><\/p><p>Division of Science and Technology, University of Education Lahore, Multan Campus 60700, Pakistan<\/p><p><span class=\"name\">Muhammad Naveed Rasheed<\/span><\/p><p>Division of Science and Technology, University of Education Lahore, Multan Campus 60700, Pakistan<\/p><p><span class=\"name\">Muhammad Idrees Jillan<\/span><\/p><p>Department of Chemistry, University of Sahiwal, Pakistan<\/p><table border=\"0\" width=\"93%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td align=\"center\" valign=\"top\"><strong>Received<\/strong><br \/>21 Feb, 2019<\/td><td align=\"center\" valign=\"top\"><strong>Accepted<\/strong><br \/>28 Mar, 2021<\/td><td align=\"center\" valign=\"top\"><strong>Published<\/strong><br \/>03 Jun, 2021<\/td><\/tr><\/tbody><\/table><div class=\"item abstract\"><p align=\"justify\">ABSTRACT:<\/p><p class=\"ql-align-justify\">The methanolic extract fractions of<em>\u00a0Malva sylvestris<\/em>, collected from Bahawalpur district, were subjected to study the total phenolic contents, total flavonoid contents, antioxidant and antimicrobial activity. The antioxidant activity was measured using the linoleic acid system, DPPH scavenging assay, and determination of reducing power. The antimicrobial activity was evaluated through the Disc diffusion method. The partition fractionation of the methanolic extract of\u00a0<em>M. sylvestris<\/em>\u00a0was performed using ethanol, acetic acid, acetone, ethyl acetate, and n-hexane. Yields of polar solvent fractions were higher than other fractions in these plants. HPLC analysis presented three major phenolics, gallic acid (141.51-192.03 \u03bcg\/g), coumaric acid (112.21-172.11 \u03bcg\/g), and Ellagic acid (110.31-159.18 \u03bcg\/g) in\u00a0<em>M. sylvestris<\/em>\u00a0with maximum yield in methanol fractions. The total phenolic contents analyzed using Folin- Ciocalteu reagent of the samples varied from 5.58- 17.1g\/100g, 2.66-7.41g\/100g dry weight expressed as gallic acid equivalents (GAE). The determined total flavonoid contents in the fractions ranged from 11.50-32.4g\/100g and 24.91-36.36g\/100g dry weight expressed as catechin equivalents. The percentage of inhibition of linoleic acid peroxidation and reducing potential was more significant in\u00a0<em>M. sylvestris<\/em>\u00a0methanol fractions, i.e., 47.6% and 3.3 mg\/100mL, respectively.\u00a0<em>M. sylvestris<\/em>\u00a0plants also demonstrated remarkable antimicrobial activity against various pathogens (<em>E.coli, P.multocida, S.aureus, B. subtillus, A. niger, A. flavus, A, fusarium, F. Solani<\/em>). The chemical screening of crude extracts constitutes an efficient complementary approach allowing localization and targeted isolation of new types of constituents with potential activities.<\/p><\/div><div class=\"item abstract\"><div><h3 class=\"label\">How to Cite this paper?<\/h3><div class=\"csl-entry\"><p><strong>APA-7 Style<\/strong><br \/>Naz , S., Rasheed, M.N., Jillan, M.I. (2021). Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0<i>Malva sylvestris (Pick-cheese)<\/i>\u00a0leaf extracts.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 1(2), 60-70<\/a>. https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02<\/p><p><strong>ACS Style<\/strong><br \/>Naz , S.; Rasheed, M.N.; Jillan, M.I. Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0<i>Malva sylvestris (Pick-cheese)<\/i>\u00a0leaf extracts.\u00a0<a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2024\/12\/ANST-v1-i2-Paper-02-2.pdf\" target=\"_blank\" rel=\"noopener\"><em>J. Adv. Nutri. Sci. Tech.<\/em>\u00a02021, 1, 60-70<\/a>. https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02<\/p><p><strong>AMA Style<\/strong><br \/>Naz S, Rasheed MN, Jillan MI. Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0<i>Malva sylvestris (Pick-cheese)<\/i>\u00a0leaf extracts.\u00a0<a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2024\/12\/ANST-v1-i2-Paper-02-2.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>. 2021; 1(2): 60-70<\/a>. https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02<\/p><p><strong>Chicago\/Turabian Style<\/strong><br \/>Naz , Saima, Muhammad Naveed Rasheed, and Muhammad Idrees Jillan. 2021. &#8220;Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0<i>Malva sylvestris (Pick-cheese)<\/i>\u00a0leaf extracts&#8221;\u00a0<a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2024\/12\/ANST-v1-i2-Paper-02-2.pdf\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>\u00a01, no. 2: 60-70<\/a>. https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02<\/p><\/div><\/div><\/div><div class=\"item copyright\"><p><a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\"><img decoding=\"async\" src=\"https:\/\/i.creativecommons.org\/l\/by\/4.0\/88x31.png\" alt=\"Creative Commons License\" \/><\/a><\/p><p>This work is licensed under a\u00a0<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\">Creative Commons Attribution 4.0 International License<\/a>.<\/p><\/div><\/div><\/div><\/article>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2f39949 e-con-full e-flex e-con e-child\" data-id=\"2f39949\" data-element_type=\"container\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Research Article | Open AccessVolume 1 | Issue 2\u00a0 | Paper 02 |\u00a0 https:\/\/doi.org\/10.15228\/ANST.2021.v01.i02.p02 Phytochemical characterization and evaluation of the antimicrobial and antioxidant activity of various fractions of\u00a0Malva sylvestris (Pick-cheese)\u00a0leaf extracts Saima Naz Division of Science and Technology, University of Education Lahore, Multan Campus 60700, Pakistan Muhammad Naveed Rasheed Division of Science and Technology, University [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[22,24],"tags":[],"class_list":["post-273","post","type-post","status-publish","format-standard","hentry","category-22","category-issue-02-2021"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Phytochemical 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