{"id":209,"date":"2022-08-10T16:18:00","date_gmt":"2022-08-10T16:18:00","guid":{"rendered":"https:\/\/chempublishers.com\/anst\/?p=209"},"modified":"2025-02-13T20:14:06","modified_gmt":"2025-02-13T20:14:06","slug":"anst-0622","status":"publish","type":"post","link":"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/2022\/08\/10\/anst-0622\/","title":{"rendered":"Determination of Aflatoxins contamination in Corn (Zea mays.) used for animal feed"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"209\" class=\"elementor elementor-209\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b50aa4a e-flex e-con-boxed e-con e-parent\" data-id=\"b50aa4a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-e16f53d e-con-full e-flex e-con e-child\" data-id=\"e16f53d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7afb377 elementor-widget elementor-widget-text-editor\" data-id=\"7afb377\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td><div><strong>Research Article | Open Access<\/strong><\/div><div class=\"item doi\"><span class=\"value\">Volume 2 | Issue 2\u00a0 | Paper 01 |\u00a0 <a href=\"https:\/\/chempublishers.com\/advances-of-nutrition-science-and-technology\/wp-content\/uploads\/sites\/3\/2024\/11\/ANST.2022.v02.i02.p01.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01<\/a><\/span><\/div><\/td><\/tr><\/tbody><\/table><article class=\"obj_article_details\"><h1 class=\"page_title\">Determination of Aflatoxins contamination in Corn (<i>Zea mays<\/i>.) used for animal feed<\/h1><div class=\"row\"><div class=\"main_entry\"><p><span class=\"name\">Alim Un Nisa<\/span><\/p><p>Food and Biotechnology Research Center, PCSIR Laboratories Complex, 54600 Lahore Pakistan<\/p><p><span class=\"name\">Phool Shahzadi<\/span><\/p><p>GCRC, PCSIR Laboratories Complex, 54600 Lahore, Pakistan<\/p><p><span class=\"name\">Sajawal Ashraf<\/span><\/p><p>Faculty of Life Sciences, University of Central Punjab Lahore, Pakistan<\/p><p><span class=\"name\">Hamood Ur Rehman<\/span><\/p><p>ACRC, PCSIR Laboratories Complex, 54600 Lahore, Pakistan<\/p><p><span class=\"name\">Shahid Masood\u00a0<\/span><\/p><p>Food and Biotechnology Research Center, PCSIR Laboratories Complex, 54600 Lahore Pakistan<\/p><p><span class=\"name\">Sajila Hina<\/span><\/p><p>Food and Biotechnology Research Center, PCSIR Laboratories Complex, 54600 Lahore Pakistan<\/p><p><span class=\"name\">Adil Hussain<\/span><\/p><p>Food and Biotechnology Research Center, PCSIR Laboratories Complex, 54600 Lahore Pakistan<\/p><table border=\"0\" width=\"93%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td align=\"center\" valign=\"top\"><strong>Received<\/strong><br \/>10 May, 2022<\/td><td align=\"center\" valign=\"top\"><strong>Accepted<\/strong><br \/>30 Jul, 2022<\/td><td align=\"center\" valign=\"top\"><strong>Published<\/strong><br \/>17 Aug, 2022<\/td><\/tr><\/tbody><\/table><div class=\"item abstract\"><p align=\"justify\">ABSTRACT:<\/p><p class=\"ql-align-justify\">This research study aimed to estimate the total aflatoxins in corn used for livestock feed intended for breeding cattle, beef, and mature poultry. Total 60 corn samples for livestock feed were collected from different markets and feed shops in Lahore. Total aflatoxin AFB1, AFB2, AFG1, and AFG2 were estimated using Thin Layer Chromatography (TLC) and quantitative ELISA methods. According to the Food and Drug Administration (FDA), the daily intake of aflatoxin in livestock feed is a 100ppb threshold. Total 60 samples were examined; 20% were contaminated with aflatoxin B1 and B2, and the rest of 55% samples were contaminated only with aflatoxin B1. Aflatoxin G1 and G2 were not detected in any feed. The results showed that 20% of samples were contaminated within the permissible limit, 55% were unfit due to having above the allowable limit, and 25% were fit, which did not show any aflatoxin. The current study has demonstrated the easy way of determining aflatoxin in corn used for livestock feed.<\/p><\/div><div class=\"item abstract\"><div><h3 class=\"label\">How to Cite this paper?<\/h3><div class=\"csl-entry\"><p><strong>APA-7 Style<\/strong><br \/>Nisa, A.U., Shahzadi, P., Ashraf, S., Rehman, H.U., Masood, S., Hina, S., Hussain, A. (2022). Determination of Aflatoxins contamination in Corn (<i>Zea mays<\/i>.) used for animal feed.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>, 2(2), 35-39<\/a>. https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01<\/p><p><strong>ACS Style<\/strong><br \/>Nisa, A.U.; Shahzadi, P.; Ashraf, S.; Rehman, H.U.; Masood, S.; Hina, S.; Hussain, A. Determination of Aflatoxins contamination in Corn (<i>Zea mays<\/i>.) used for animal feed.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01\" target=\"_blank\" rel=\"noopener\"><em>J. Adv. Nutri. Sci. Tech.<\/em>\u00a02022, 2, 35-39<\/a>. https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01<\/p><p><strong>AMA Style<\/strong><br \/>Nisa AU, Shahzadi P, Ashraf S, Rehman HU, Masood S, Hina S, Hussain A. Determination of Aflatoxins contamination in Corn (<i>Zea mays<\/i>.) used for animal feed.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>. 2022; 2(2): 35-39<\/a>. https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01<\/p><p><strong>Chicago\/Turabian Style<\/strong><br \/>Nisa, Alim, Un, Phool Shahzadi, Sajawal Ashraf, Hamood Ur Rehman, Shahid Masood, Sajila Hina, and Adil Hussain. 2022. &#8220;Determination of Aflatoxins contamination in Corn (<i>Zea mays<\/i>.) used for animal feed&#8221;\u00a0<a href=\"https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01\" target=\"_blank\" rel=\"noopener\"><em>Journal Advances of Nutrition Science and Technology<\/em>\u00a02, no. 2: 35-39<\/a>. https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01<\/p><\/div><\/div><\/div><div class=\"item copyright\"><p><a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\"><img decoding=\"async\" src=\"https:\/\/i.creativecommons.org\/l\/by\/4.0\/88x31.png\" alt=\"Creative Commons License\" \/><\/a><\/p><p>This work is licensed under a\u00a0<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\">Creative Commons Attribution 4.0 International License<\/a>.<\/p><\/div><\/div><\/div><\/article>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-5422f60 e-con-full e-flex e-con e-child\" data-id=\"5422f60\" data-element_type=\"container\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Research Article | Open AccessVolume 2 | Issue 2\u00a0 | Paper 01 |\u00a0 https:\/\/doi.org\/10.15228\/ANST.2022.v02.i02.p01 Determination of Aflatoxins contamination in Corn (Zea mays.) used for animal feed Alim Un Nisa Food and Biotechnology Research Center, PCSIR Laboratories Complex, 54600 Lahore Pakistan Phool Shahzadi GCRC, PCSIR Laboratories Complex, 54600 Lahore, Pakistan Sajawal Ashraf Faculty of Life Sciences, [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[18,20],"tags":[],"class_list":["post-209","post","type-post","status-publish","format-standard","hentry","category-18","category-issue-02"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Determination of Aflatoxins contamination in Corn (Zea mays.) used for animal feed - The Journal Advances of Nutrition Science and Technology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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