{"id":2076,"date":"2011-04-20T10:22:00","date_gmt":"2011-04-20T10:22:00","guid":{"rendered":"https:\/\/chempublishers.com\/pjc\/?p=2076"},"modified":"2025-03-20T14:59:01","modified_gmt":"2025-03-20T14:59:01","slug":"pjc-1211","status":"publish","type":"post","link":"https:\/\/chempublishers.com\/Pakistan-journal-of-chemistry\/2011\/04\/20\/pjc-1211\/","title":{"rendered":"Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2076\" class=\"elementor elementor-2076\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3abc23d e-flex e-con-boxed e-con e-parent\" data-id=\"3abc23d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-4b5214d e-con-full e-flex e-con e-child\" data-id=\"4b5214d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-20e17d2 elementor-widget elementor-widget-text-editor\" data-id=\"20e17d2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td><div><strong>Research Article | Open Access<\/strong><\/div><div class=\"item doi\"><span class=\"value\">Volume 1 | Issue 2 |\u00a0<a href=\"https:\/\/chempublishers.com\/Pakistan-journal-of-chemistry\/wp-content\/uploads\/sites\/2\/2025\/01\/PJC-v01.i02.p04.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.15228\/2011.v01.i02.p04<\/a><\/span><\/div><\/td><\/tr><\/tbody><\/table><article class=\"obj_article_details\"><h4 class=\"page_title\">Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals<\/h4><div class=\"row\"><div class=\"main_entry\"><p><span class=\"name\">R.H. Jasim<\/span><\/p><p>Chemistry Department, College of Education for Girls, University of Kufa<\/p><p><span class=\"name\">H.R. Hasan<\/span><\/p><p>Chemistry Department, College of Science, University of Baghdad.<\/p><p><span class=\"name\">M.K. Husain<\/span><\/p><p>Biochemistry Department, College of Medicine, University of Kufa<\/p><table border=\"0\" width=\"93%\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\"><tbody><tr><td align=\"center\" valign=\"top\"><strong>Received<\/strong><br \/>11 Mar, 2011<\/td><td align=\"center\" valign=\"top\"><strong>Accepted<\/strong><br \/>11 Jun, 2011<\/td><td align=\"center\" valign=\"top\"><strong>Published<\/strong><br \/>11 Dec, 2011<\/td><\/tr><\/tbody><\/table><div class=\"item abstract\"><p class=\"ql-align-justify\">The present study was designed to investigate the presence of mannose binding lectin (ManBL) in the sera and tissue samples of patients with tumoral (benign &amp; malignant) kidney in addition to patients with non tumoral kidney affection, in order to establish the possibility of using serum and tissue ManBL test for diagnosis and epidemiological testing of kidney cancers. Participants of the present study were 96 patients at the age range 12-80 years; they were attending by different hospitals in Najaf (basically) and Karbala, and 46 healthy individuals within the same age range of the studying patients groups. Specific hemagglutination activity levels were revealed a significant increased (p&lt;0.001) in the sera and tissues of the patients with the malignant tumors specimens in comparison to that of pathological controls and healthy individuals. Serum and tissue hemagglutination activity levels were significantly higher (p&lt;0.001) in patients with metastatic disease compared with patients with localized tumors. While, the levels of the hemagglutination activity were approximate at patients with the end malignant kidney tumors stages. Upon electrophoresis of the study samples, the results reflected presence of changes in some proteins and glycoproteins bands presents in these samples. Using hydrophobic affinity chromatography, ManBLs were purified from sera of malignant, benign kidney tumors and non tumoral kidney diseases in addition to healthy individuals groups. The purified lectins were found to be glycoprotein with 77.45 KD as an approximate molecular weight and its sugar content was equal to13.5%. The maximum concentration of purified ManBL was found at patients with malignant kidney tumors.The results revealed that the highest hemagglutination activity of purified ManBL occurred with O<sup>+<\/sup>\u00a0RBCs, at pH 7.4, and 37\u02daC. ManBL (regardless of its origin) lost the hemagglutination activity, completely, in the presence of EDTA. A result that indicates that the present purified the human ManBLs is a calcium dependent type.<\/p><\/div><div class=\"item abstract\"><div><h3 class=\"label\">How to Cite this paper?<\/h3><div class=\"csl-entry\"><p><strong>APA-7 Style<\/strong><br \/>Jasim, R.H., Hasan, H.R., Husain , M.K. (2011). Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals<br \/>.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/2011.v01.i02.p04\" target=\"_blank\" rel=\"noopener\"><em>Pakistan Journal of Chemistry<\/em>, 1(2), 72-82<\/a>. https:\/\/doi.org\/10.15228\/2011.v01.i02.p04<\/p><p><strong>ACS Style<\/strong><br \/>Jasim, R.H.; Hasan, H.R.; Husain , M.K. Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals<br \/>.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/2011.v01.i02.p04\" target=\"_blank\" rel=\"noopener\"><em>Pak. J. Chem.<\/em>\u00a02011, 1, 72-82<\/a>. https:\/\/doi.org\/10.15228\/2011.v01.i02.p04<\/p><p><strong>AMA Style<\/strong><br \/>Jasim RH, Hasan HR, Husain MK. Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals<br \/>.\u00a0<a href=\"https:\/\/doi.org\/10.15228\/2011.v01.i02.p04\" target=\"_blank\" rel=\"noopener\"><em>Pakistan Journal of Chemistry<\/em>. 2011; 1(2): 72-82<\/a>. https:\/\/doi.org\/10.15228\/2011.v01.i02.p04<\/p><p><strong>Chicago\/Turabian Style<\/strong><br \/>Jasim, R., H., H. R. Hasan, and M. K. Husain . 2011. &#8220;Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals<br \/>&#8221;\u00a0<a href=\"https:\/\/doi.org\/10.15228\/2011.v01.i02.p04\" target=\"_blank\" rel=\"noopener\"><em>Pakistan Journal of Chemistry<\/em>\u00a01, no. 2: 72-82<\/a>. https:\/\/doi.org\/10.15228\/2011.v01.i02.p04<\/p><\/div><\/div><\/div><div class=\"item copyright\"><p><a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\"><img decoding=\"async\" src=\"https:\/\/i.creativecommons.org\/l\/by\/4.0\/88x31.png\" alt=\"Creative Commons License\" \/><\/a><\/p><p>This work is licensed under a\u00a0<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" rel=\"license\">Creative Commons Attribution 4.0 International License<\/a>.<\/p><\/div><\/div><\/div><\/article>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6a71383 e-con-full e-flex e-con e-child\" data-id=\"6a71383\" data-element_type=\"container\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Research Article | Open AccessVolume 1 | Issue 2 |\u00a0https:\/\/doi.org\/10.15228\/2011.v01.i02.p04 Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals R.H. Jasim Chemistry Department, College of Education for Girls, University of Kufa H.R. Hasan Chemistry Department, College of Science, University of Baghdad. M.K. Husain Biochemistry Department, [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-2076","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals - Pakistan Journal of Chemistry<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/chempublishers.com\/Pakistan-journal-of-chemistry\/2011\/04\/20\/pjc-1211\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals - Pakistan Journal of Chemistry\" \/>\n<meta property=\"og:description\" content=\"Research Article | Open AccessVolume 1 | Issue 2 |\u00a0https:\/\/doi.org\/10.15228\/2011.v01.i02.p04 Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals R.H. 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